Description
Aviva Systems Biology UBE2I ELISA Kit (Human) (OKEH02141) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UBE2I has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UBE2I is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample UBE2I captured in well